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Mg and Al-Mg Inhibit Migration and Invasion of Hepatocellular and Pancreatic Cancer Cells In Vitro . (A) Schematic diagram of scratch assays with tumor cells treated by Mg rods and Al-Mg rods. (B) Scratch assays showing reduced migration abilities of hepatocellular cancer cells (Huh7, PLC/PRF/5) and pancreatic cancer cells (PANC-1, Capan-2) after Mg and Al-Mg treatments. (C) Schematic diagram of Transwell assays with tumor cells treated by Mg rods and Al-Mg rods. (D) Transwell migration assays demonstrating decreased migration of hepatocellular cancer cells (Huh7, PLC/PRF/5) and pancreatic cancer cells (PANC-1, Capan-2) following Mg and Al-Mg treatments. (E) Transwell invasion assays showing reduced invasion capabilities of hepatocellular cancer cells (Huh7, PLC/PRF/5) and pancreatic cancer cells (PANC-1, Capan-2) after Mg and Al-Mg treatments. (F) Immunofluorescence analysis showing <t>decreased</t> <t>N-cadherin</t> expression in PANC-1 and Huh7 cells after Mg and Al-Mg treatments. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
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Mg and Al-Mg Inhibit Migration and Invasion of Hepatocellular and Pancreatic Cancer Cells In Vitro . (A) Schematic diagram of scratch assays with tumor cells treated by Mg rods and Al-Mg rods. (B) Scratch assays showing reduced migration abilities of hepatocellular cancer cells (Huh7, PLC/PRF/5) and pancreatic cancer cells (PANC-1, Capan-2) after Mg and Al-Mg treatments. (C) Schematic diagram of Transwell assays with tumor cells treated by Mg rods and Al-Mg rods. (D) Transwell migration assays demonstrating decreased migration of hepatocellular cancer cells (Huh7, PLC/PRF/5) and pancreatic cancer cells (PANC-1, Capan-2) following Mg and Al-Mg treatments. (E) Transwell invasion assays showing reduced invasion capabilities of hepatocellular cancer cells (Huh7, PLC/PRF/5) and pancreatic cancer cells (PANC-1, Capan-2) after Mg and Al-Mg treatments. (F) Immunofluorescence analysis showing decreased <t>N-cadherin</t> expression in PANC-1 and Huh7 cells after Mg and Al-Mg treatments. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
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Mg and Al-Mg Inhibit Migration and Invasion of Hepatocellular and Pancreatic Cancer Cells In Vitro . (A) Schematic diagram of scratch assays with tumor cells treated by Mg rods and Al-Mg rods. (B) Scratch assays showing reduced migration abilities of hepatocellular cancer cells (Huh7, PLC/PRF/5) and pancreatic cancer cells (PANC-1, Capan-2) after Mg and Al-Mg treatments. (C) Schematic diagram of Transwell assays with tumor cells treated by Mg rods and Al-Mg rods. (D) Transwell migration assays demonstrating decreased migration of hepatocellular cancer cells (Huh7, PLC/PRF/5) and pancreatic cancer cells (PANC-1, Capan-2) following Mg and Al-Mg treatments. (E) Transwell invasion assays showing reduced invasion capabilities of hepatocellular cancer cells (Huh7, PLC/PRF/5) and pancreatic cancer cells (PANC-1, Capan-2) after Mg and Al-Mg treatments. (F) Immunofluorescence analysis showing decreased N-cadherin expression in PANC-1 and Huh7 cells after Mg and Al-Mg treatments. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

Journal: Bioactive Materials

Article Title: A promising magnesium-related alloy with metabolic reprogramming and antitumor effects in hepatocellular and pancreatic cancer

doi: 10.1016/j.bioactmat.2025.12.039

Figure Lengend Snippet: Mg and Al-Mg Inhibit Migration and Invasion of Hepatocellular and Pancreatic Cancer Cells In Vitro . (A) Schematic diagram of scratch assays with tumor cells treated by Mg rods and Al-Mg rods. (B) Scratch assays showing reduced migration abilities of hepatocellular cancer cells (Huh7, PLC/PRF/5) and pancreatic cancer cells (PANC-1, Capan-2) after Mg and Al-Mg treatments. (C) Schematic diagram of Transwell assays with tumor cells treated by Mg rods and Al-Mg rods. (D) Transwell migration assays demonstrating decreased migration of hepatocellular cancer cells (Huh7, PLC/PRF/5) and pancreatic cancer cells (PANC-1, Capan-2) following Mg and Al-Mg treatments. (E) Transwell invasion assays showing reduced invasion capabilities of hepatocellular cancer cells (Huh7, PLC/PRF/5) and pancreatic cancer cells (PANC-1, Capan-2) after Mg and Al-Mg treatments. (F) Immunofluorescence analysis showing decreased N-cadherin expression in PANC-1 and Huh7 cells after Mg and Al-Mg treatments. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

Article Snippet: Subsequently, diluted primary antibody N-cadherin (1:500, Proteintech, 22018-1-AP) was added, and cells were incubated overnight at 4 °C.

Techniques: Migration, In Vitro, Immunofluorescence, Expressing

Mg and Al-Mg Inhibit Migration and Invasion of Hepatocellular and Pancreatic Cancer Cells In Vitro . (A) Schematic diagram of scratch assays with tumor cells treated by Mg rods and Al-Mg rods. (B) Scratch assays showing reduced migration abilities of hepatocellular cancer cells (Huh7, PLC/PRF/5) and pancreatic cancer cells (PANC-1, Capan-2) after Mg and Al-Mg treatments. (C) Schematic diagram of Transwell assays with tumor cells treated by Mg rods and Al-Mg rods. (D) Transwell migration assays demonstrating decreased migration of hepatocellular cancer cells (Huh7, PLC/PRF/5) and pancreatic cancer cells (PANC-1, Capan-2) following Mg and Al-Mg treatments. (E) Transwell invasion assays showing reduced invasion capabilities of hepatocellular cancer cells (Huh7, PLC/PRF/5) and pancreatic cancer cells (PANC-1, Capan-2) after Mg and Al-Mg treatments. (F) Immunofluorescence analysis showing decreased N-cadherin expression in PANC-1 and Huh7 cells after Mg and Al-Mg treatments. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

Journal: Bioactive Materials

Article Title: A promising magnesium-related alloy with metabolic reprogramming and antitumor effects in hepatocellular and pancreatic cancer

doi: 10.1016/j.bioactmat.2025.12.039

Figure Lengend Snippet: Mg and Al-Mg Inhibit Migration and Invasion of Hepatocellular and Pancreatic Cancer Cells In Vitro . (A) Schematic diagram of scratch assays with tumor cells treated by Mg rods and Al-Mg rods. (B) Scratch assays showing reduced migration abilities of hepatocellular cancer cells (Huh7, PLC/PRF/5) and pancreatic cancer cells (PANC-1, Capan-2) after Mg and Al-Mg treatments. (C) Schematic diagram of Transwell assays with tumor cells treated by Mg rods and Al-Mg rods. (D) Transwell migration assays demonstrating decreased migration of hepatocellular cancer cells (Huh7, PLC/PRF/5) and pancreatic cancer cells (PANC-1, Capan-2) following Mg and Al-Mg treatments. (E) Transwell invasion assays showing reduced invasion capabilities of hepatocellular cancer cells (Huh7, PLC/PRF/5) and pancreatic cancer cells (PANC-1, Capan-2) after Mg and Al-Mg treatments. (F) Immunofluorescence analysis showing decreased N-cadherin expression in PANC-1 and Huh7 cells after Mg and Al-Mg treatments. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

Article Snippet: Primary antibodies used included p-AMPK (1:100, CST, 2535), Ki-67 (1:1000, Servicebio, GB111499-50), E-cadherin (1:500, Servicebio, GB12083-100), N-cadherin (1:500, Proteintech, SA00013-4), and Vimentin (1:2000, Servicebio, GB11192-100).

Techniques: Migration, In Vitro, Immunofluorescence, Expressing